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Santa Cruz Biotechnology
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Journal: bioRxiv
Article Title: Soil microbiome structure and function reflect environmental variation rather than reindeer presence in a northern peatland
doi: 10.64898/2026.05.13.724277
Figure Lengend Snippet: A) Relative abundance and expression of the key nitrogen-cycling marker genes and their functional ratios in metagenomic (MG) and metatranscriptomic (MT) data comparing plots outside and inside the exclusion fence. B) Relative abundance and expression of the methane-cycling marker genes and the methane oxidation/methanogenesis ratio in MG and MT data. C) September pore-water nitrate + nitrite (NO₃⁻ + NO₂⁻) and total nitrogen (Total N) by the exclusion treatment and vegetation cluster with the associated ( nirS + nirK)/nosZ ratios in MG and MT data. D) Methane fluxes measured near the soil sampling dates by the exclusion treatment and vegetation cluster and the associated pmoA/mcrA ratios in MG and MT data. E) Linear regressions showing associations between pore-water NO₃⁻ + NO₂⁻ and the denitrification genes/ratios, methane fluxes and methane oxidation/methanogenesis genes/ratios. Abbreviations for the vegetation clusters, T.ces = Trichophorum cespitosum , C.ros = Carex rostrata , C.cho = Carex chordorrhiza . MG and MT reads were aligned to the eukaryote-filtered Greening Lab metabolic marker database and summarized to marker-gene level. Marker gene counts were converted to copies-per-million (as for transcripts-per-million). Linear mixed-effects models (LMMs) (exclusion treatment and snow treatment as fixed effects, vegetation cluster as a random effect) were used to determine significance of the MG abundance and MT expression association to the treatments. For the LMMs the marker gene data were log₂-transformed. Pore-water NO₃⁻ + NO₂⁻, Total N, methane fluxes and functional ratios were natural-log transformed for the regression analyses. Boxplots are plotted without potential outlier observations for better visualization.
Article Snippet:
Techniques: Expressing, Marker, Functional Assay, Sampling, Transformation Assay